褪黑素调节雄性大鼠脂肪代谢的研究

doi:10.11843/j.issn.0366-6964.2017.09.014

Abstract

Abstract:

The present study aimed to investigate the effect of melatonin (MT) on lipid metabolism and its effect on lipid metabolism enzymes and factors, and to reveal the mechanism of lipid metabolism in male rats. Twenty SD male rats with similar age and body weight were randomly divided into 2 groups (10 rats per group),which were control group (normal diet, ND) and MT treated group (normal diet with oral melatonin solution, ND+MT). The MT was administrated at 10 mg·kg^-1 BW per day.The experiment lasted for 60 days. At the end of the experiment, blood sample were collected to extract plasma to detect biochemical, hormonal and lipid metabolism related indicators; The rats were sacrificed, the liver and the adipose mass in the abdomen, such as omentum, perirenal fat and epididymal fat was separated and weighed. In additon, liver tissue was sampled for Gene chip and related molecules analysis. The result showed that:1) The liver weight, liver index, omental weight, omental index and epididymal fat weight in ND + MT group were decreased significantly (P<0.05). Low density lipoprotein in serum was significantly decreased (P<0.05), high density lipoprotein and total cholesterol in serum were decreased, while very low density lipoprotein increased.The contents of hepatic long chain fatty acids (LCFA) was significantly decreased (P<0.05). 2) Gene chip and fluorescent quantitative PCR result showed that the mRNA expression of lipolysis related factors and enzymes, such as CPT1(P<0.05) and ALDH3a2 (0.05 < P < 0.1) in liver tissue were increased, while the mRNA expression of fat synthesis related enzyme, such as ACSL3 and FAS was decreased significantly (P<0.05). MT can greatly affect the lipid metabolism with the reduction of visceral fat, its mechanism is connected with the response of hormones and lipid metabolism related enzymes or factors to MT.

CLC Number:

S852.2

YAN Ling-min, YANG Gai-qing, WANG Lin-feng, CHEN Yu, ZHU He-shui, GAO Teng-yun, LI Ming. The Study of Melatonin Regulating Lipid Metabolism in Male Rats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1694-1704.

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The Study of Melatonin Regulating Lipid Metabolism in Male Rats

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